Activating Compound | Comment | Organism | Structure |
---|---|---|---|
1,4-dioxane | 3.3fold activation at 2.5% v/v | Streptomyces rimosus | |
acetone | 1.9fold activation at 2.5% v/v | Streptomyces rimosus | |
dimethyl sulfoxide | 1.4fold activation at 2.5% v/v | Streptomyces rimosus | |
ethanol | 1.3fold activation at 2.5% v/v | Streptomyces rimosus | |
N,N-Dimethylformamide | 1.8fold activation at 2.5% v/v | Streptomyces rimosus | |
tetrahydrofuran | 2.9fold activation at 2.5% v/v | Streptomyces rimosus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Streptomyces rimosus | - |
- |
Organic Solvent | Comment | Organism |
---|---|---|
1,4-dioxane | 18 h at 50% v/v, 100% stable | Streptomyces rimosus |
Acetone | 18 h at 50% v/v, 80% remaining activity | Streptomyces rimosus |
acetonitrile | 18 h at 50% v/v, 92% remaining activity | Streptomyces rimosus |
dimethyl sulfoxide | 18 h at 50% v/v, 63% remaining activity | Streptomyces rimosus |
Ethanol | 18 h at 50% v/v, 91% remaining activity | Streptomyces rimosus |
N,N-dimethylformamide | 18 h at 50% v/v, 4% remaining activity | Streptomyces rimosus |
tetrahydrofuran | 18 h at 50% v/v, no remaining activity, complete inactivation | Streptomyces rimosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces rimosus | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
170 | - |
purified enzyme, substrate triolein | Streptomyces rimosus |
203 | - |
purified enzyme, substrate poly(oxyethylene) sorbitan monooleate | Streptomyces rimosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3-dimercapto-1-propanol tributyrate + H2O | regiospecificity study | Streptomyces rimosus | ? | - |
? | |
additional information | enzyme also performs transesterification reactions, regio- and enantioselctivity, overview, substrate specificity: enzyme hydrolyzes most efficiently medium chain length fatty acid glycerol esters, C8-C12, and shows a preference for esters of C16 and C18 unsaturated fatty acids over C16 and C18 saturated fatty acid esters, as well as for triacylglycerol substrates with cis double bond, e.g. triolein, versus trans double bonds, e.g. trielaidin, enzyme also utilizes different natural plant oils as substrates, overview | Streptomyces rimosus | ? | - |
? | |
tributyrin + H2O | - |
Streptomyces rimosus | dibutyrin + butyrate | - |
? | |
tricaprin + H2O | best substrate | Streptomyces rimosus | dicaprin + capriate | - |
? | |
tricaprylin + H2O | best substrate | Streptomyces rimosus | dicaprylin + caprylate | - |
? | |
trielaidin + H2O | trans C18:1 | Streptomyces rimosus | dielaidin + elaidiate | - |
? | |
trilinolein + H2O | - |
Streptomyces rimosus | dilinolein + linoleate | - |
? | |
trilinolenin + H2O | - |
Streptomyces rimosus | dilinolenin + linolenate | - |
? | |
triolein + H2O | cis C18:1 | Streptomyces rimosus | diolein + oleate | main product is oleic acid, further products are 1,3-dioleylglycerol, 1,2-dioleylglycerol, 2,3-dioleylglycerol, and minor product is 1-monooleylglycerol | ? | |
tripalmitin + H2O | - |
Streptomyces rimosus | dipalmitin + palmitate | - |
? | |
tripalmitolein + H2O | - |
Streptomyces rimosus | dipalmitolein + palmitate | - |
? | |
tristearin + H2O | - |
Streptomyces rimosus | distearin + stearate | - |
? | |
Tween 20 + H2O | i.e. poly(oxyethylene) sorbitan monolaurate | Streptomyces rimosus | ? | - |
? | |
Tween 40 + H2O | i.e. poly(oxyethylene) sorbitan monopalmitate | Streptomyces rimosus | ? | - |
? | |
Tween 60 + H2O | i.e. poly(oxyethylene) sorbitan monostearate | Streptomyces rimosus | ? | - |
? | |
Tween 80 + H2O | i.e. poly(oxyethylene) sorbitan monooleate | Streptomyces rimosus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
lipase | - |
Streptomyces rimosus |